If my antibody isn't working as intended, what should I do?

Verify the assay methods that are appropriate, the expiration date, and the storage circumstances. Modify antibody concentrations, lengthen incubation times, and include appropriate controls in your studies.

How can I make sure this antibody is stable over time?

To avoid repeated freeze-thaw cycles, aliquot the antibody solution, store the antibodies properly, and limit light and temperature exposure.

For my experiment, how do I choose the appropriate secondary antibody?

Choose a detection approach that meets your experiment's needs, as well as a secondary antibody specific to the host species and isotope of your primary antibody.

Recombinant Human Anti-EGFR Antibody (PABX-052)

CAT#: PABX-052

Anti-EGFR antibody is a Humanized antibody of IgG class that binds to an EGFR.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Recombinant Human Anti-EGFR Antibody (PABX-052) in SDS-PAGE

SDS-PAGE analysis of PABX-052 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of PABX-052 was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 2 Recombinant Human Anti-EGFR Antibody (PABX-052) in HPLC

The purity of PABX-052 was greater than 95% as determined by SEC-HPLC.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG

Specificity
Tested positive against native Human EGFR

Species Reactivity
Human

Applications
WB, ELISA, FuncS, IB, FC, SPR, Apop

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
The EGFR antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoblotting, Flow Cytometry, Surface Plasmon Resonance, Apoptosis.

Target

Alternative Names
EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; mAb806; LICR 806; ABT-806; ch806

Gene ID
1956

UniProt ID
P00533

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Q&As

If my antibody isn't working as intended, what should I do?

A: Verify the assay methods that are appropriate, the expiration date, and the storage circumstances. Modify antibody concentrations, lengthen incubation times, and include appropriate controls in your studies.
How can I make sure this antibody is stable over time?

A: To avoid repeated freeze-thaw cycles, aliquot the antibody solution, store the antibodies properly, and limit light and temperature exposure.
For my experiment, how do I choose the appropriate secondary antibody?

A: Choose a detection approach that meets your experiment's needs, as well as a secondary antibody specific to the host species and isotope of your primary antibody.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABX-052, RRID: AB_3111696)

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Biosimilar Overview

Please refer to Nimotuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Nimotuzumab.

Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-052.pdf

MSDS

MSDS - PABX-052.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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CAT	Product Name	Application	Type
PSBL-080	Human Anti-EGFR Recombinant Antibody; scFv Fragment (PSBL-080)	ELISA, WB, FuncS	Human scFv
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HPAB-2195LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2195LY-S(P))	ELISA	Mouse scFv
HPAB-2196LY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2196LY-S(P))	ELISA	Mouse scFv
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CAT	Product Name	Application	Type
TAB-0225CL	Human Anti-EGFR Recombinant Antibody (TAB-0225CL)	Block, Inhib, FuncS, Apop, In vivo	Chimeric (Mouse/Human) IgG1
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TAB-308MZ	Human Anti-EGFR Recombinant Antibody (TAB-308MZ)	FuncS	Chimeric antibody (mouse/human)
TAB-272MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P))	FuncS	Mouse scFv
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CAT	Product Name	Application	Type
TAB-297MZ-S(P)	Anti-Human EGFR Recombinant Antibody scFv Fragment (H225)	ELISA, WB	Humanized antibody
TAB-298MZ-S(P)	Anti-Human EGFR Recombinant Antibody scFv Fragment (Hu225)	ELISA, WB	Humanized antibody
TAB-300MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-300MZ-S(P))	ELISA	Humanized scFv
TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab
TAB-297MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (H225)	ELISA, WB	Humanized antibody

CAT	Product Name	Application	Type
TAB-273MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-273MZ-F(E))	FC	Mouse Fab
TAB-275MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-275MZ-F(E))	FC	Mouse Fab
TAB-276MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (LA1)	MTT assay
TAB-277MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (LA22)	MTT assay
TAB-281MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-281MZ-F(E))	WB, IF, IHC	Mouse Fab

CAT	Product Name	Application	Type
TAB-278MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-278MZ-F(E))	ELISA, WB	Human Fab
TAB-279MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-279MZ-F(E))	ELISA, WB	Human Fab
TAB-282MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-282MZ-F(E))	ELISA	Human Fab
TAB-283MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-283MZ-F(E))	ELISA	Human Fab
TAB-284MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-284MZ-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM
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CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ)	IF, FC, WB, IP, Neut	Rabbit IgG

CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
MOR-4570	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS)	ELISA	Rabbit IgG
MOR-4571	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-0033-FY	Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004)	ICC, IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-464CQ)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-003)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-119)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-2194LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E))	ELISA, WB	Mouse Fab
HPAB-2195LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E))	ELISA	Mouse Fab
HPAB-2196LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E))	ELISA	Mouse Fab
HPAB-2197LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2197LY-F(E))	ELISA	Mouse Fab
HPAB-2198LY-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2198LY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP881-YC)	ELISA, FC, IP, FuncS	Llama VHH
HPAB-AP882-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP882-YC)	ELISA	Llama VHH
HPAB-AP883-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP883-YC)	ELISA	Llama VHH
HPAB-AP884-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP884-YC)	ELISA	Llama VHH
HPAB-AP885-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP885-YC)	ELISA	Llama VHH

CAT	Product Name	Application	Type
VS-0424-XY84	AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC32	Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1
VS-0924-YC35	Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker	IHC, WB, IF	Rabbit IgG

CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

CAT	Product Name	Application	Type
VS-0125-FY28	Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera	FC, Cyt	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY102	CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102)	FC	Mouse IgG1, kappa

CAT	Product Name	Application
VS-0325-XY735	Anti-EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2183	Anti-Mouse EGFR Immunohistochemistry Kit	IHC
VS-0525-XY2182	Anti-Human EGFR Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC340	Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC65	Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3)	FC	Tandem scFv
VS-0525-YC66	Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1)	FC	Tandem scFv
VS-0525-YC68	Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5)	FC	Tandem scFv
VS-0525-YC213	Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IHC, IP	Tandem scFv

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